Studying RecA Homology Search Mechanism using Single-Molecule Methods
نویسندگان
چکیده
منابع مشابه
Direct Single-Molecule Observation of Mode and Geometry of RecA-Mediated Homology Search.
Genomic integrity, when compromised by accrued DNA lesions, is maintained through efficient repair via homologous recombination. For this process the ubiquitous recombinase A (RecA), and its homologues such as the human Rad51, are of central importance, able to align and exchange homologous sequences within single-stranded and double-stranded DNA in order to swap out defective regions. Here, we...
متن کاملOn the Mechanism of Homology Search by RecA Protein Filaments.
Genetic stability is a key factor in maintaining, survival, and reproduction of biological cells. It relies on many processes, but one of the most important is a homologous recombination, in which the repair of breaks in double-stranded DNA molecules is taking place with a help of several specific proteins. In bacteria, this task is accomplished by RecA proteins that are active as nucleoprotein...
متن کاملThe mechanism of the search for homology promoted by recA protein. Facilitated diffusion within nucleoprotein networks.
recA protein promotes the homologous pairing of single strands with duplex DNA by polymerizing on the single strands to make presynaptic nucleoprotein filaments which are polyvalent with respect to duplex DNA and which consequently form large networks or coaggregates when duplex DNA is added. Previous work has shown that efficient homologous pairing occurs within these networks. In the experime...
متن کاملHomology Search Methods
Homology search methods have advanced substantially in recent years. Beginning with the elegant Needleman-Wunsch and Smith-Waterman dynamic programming techiques of the 1970s, algorithms have been developed that were appropriate for the data sets and computer systems of their times. As data sets grew, faster but less sensitive heuristic algorithms, such as FASTA and BLAST, became a dominant for...
متن کاملRecA filament sliding on DNA facilitates homology search
During homologous recombination, RecA forms a helical filament on a single stranded (ss) DNA that searches for a homologous double stranded (ds) DNA and catalyzes the exchange of complementary base pairs to form a new heteroduplex. Using single molecule fluorescence imaging tools with high spatiotemporal resolution we characterized the encounter complex between the RecA filament and dsDNA. We p...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.1551